Peptidomic profiling of cerebrospinal fluid from patients with intracranial saccular aneurysms.

Intracranial saccular aneurysms (ISA) represent 90%-95% of all intracranial aneurysm cases, characterizing abnormal pockets at arterial branch points. Ruptures lead to subarachnoid hemorrhages (SAH) and poor prognoses. We applied mass spectrometry-based peptidomics to investigate the peptidome of twelve cerebrospinal fluid (CSF) samples collected from eleven patients diagnosed with ISA. For peptide profile analyses, participants were classified into: 1) ruptured intracranial saccular aneurysms (RIA), 2) unruptured intracranial saccular aneurysms (UIA), and late-ruptured intracranial saccular aneurysms (LRIA). Altogether, a total of 2199 peptides were detected by both Mascot and Peaks software, from which 484 (22.0%) were unique peptides. All unique peptides presented conserved chains, domains, regions of protein modulation and/or post-translational modification sites related to human diseases. Gene Ontology (GO) analyses of peptide precursor proteins showed that 42% are involved in binding, 56% in cellular anatomical entities, and 39% in intercellular signaling molecules. Unique peptides identified in patients diagnosed with RIA have a larger molecular weight and a distinctive developmental process compared to UIA and LRIA (P ≤ 0.05). Continued investigations will allow the characterization of the biological and clinical significance of the peptides identified in the present study, as well as identify prototypes for peptide-based pharmacological therapies to treat ISA. SIGNIFICANCE.

Evaluation of 2 Low-cost High-definition Video Systems for Venous Anastomosis.

Background. A literature review of the use of video systems for magnification has suggested that so far, this novel magnification system has only been used to perform arterial anastomoses. The aim of this study was to evaluate the use of 2 low-cost methods of video-assisted magnification in microvascular venous anastomosis in rats. Methods. Thirty rats were randomly divided into 3 matched groups according to the magnification system used: the microscope group, with venous anastomosis performed under a microscope; the camcorder system group, with the procedures performed under a high-definition Handycam HDR-XR160; and the Photographic camera group, for which procedures were performed with an EOS Rebel T3i photographic camera. In both video system groups, a magnification system was connected to a 42-in. television by an HDMI cable. We analyzed weight, venous caliber, total surgery and anastomosis time, patency immediately and 14 days postoperatively, number of stitches, and histological analyses. Results. There were no significant differences between the groups in weight, venous caliber, or number of stitches. Anastomosis under the video systems took longer. Patency rates were similar between the groups, except for the photographic system group that has a lower patency rate at 14 days. The histological analyses were similar in all groups. Conclusion. It is possible to perform a venous anastomosis in rats through video system magnification, with a satisfactory success rate comparable with that for procedures performed under microscopes; however, the kind of video system has a great influence on the final patency.

Citations of Brazilian physical therapy journals in national publications.

BACKGROUND: Quotations in Brazilian journals are mainly obtained from national articles (articles from Brazilian journals); thus, it is essential to determine how frequently these articles reference Brazilian journals. OBJECTIVE: This study sought to verify how frequently national papers are cited in the references of three Brazilian physical therapy journals. METHOD: All references for articles published in Fisioterapia em Movimento, Fisioterapia e Pesquisa and Revista Brasileira de Fisioterapia between 2010 and 2012 were evaluated. In particular, the numbers of national articles and international articles (articles from international journals) cited in these references were determined. RESULTS: A total of 13,009 references cited by 456 articles were analyzed, and 2,924 (22.47%) of the cited works were national articles. There were no significant differences among the three examined years. A total of 36 (7.89%) articles did not cite national articles, whereas 65 (13.25%) articles cited more national articles than international articles. CONCLUSION: On average, 22.47% of the works cited by the evaluated articles were national articles. No significant differences were detected among the three analyzed years.

Citations of Brazilian physical therapy journals in national publications / Citacao de periodicos de fisioterapia brasileiros em publicacoes nacionais

Background: Quotations in Brazilian journals are mainly obtained from national articles (articles from Brazilian journals); thus, it is essential to determine how frequently these articles reference Brazilian journals. Objective: This study sought to verify how frequently national papers are cited in the references of three Brazilian physical therapy journals. Method: All references for articles published in Fisioterapia em Movimento, Fisioterapia e Pesquisa and Revista Brasileira de Fisioterapia between 2010 and 2012 were evaluated. In particular, the numbers of national articles and international articles (articles from international journals) cited in these references were determined. Results: A total of 13,009 references cited by 456 articles were analyzed, and 2,924 (22.47%) of the cited works were national articles. There were no significant differences among the three examined years. A total of 36 (7.89%) articles did not cite national articles, whereas 65 (13.25%) articles cited more national articles than international articles. Conclusion: On average, 22.47% of the works cited by the evaluated articles were national articles. No significant differences were detected among the three analyzed years. .

Contextualização: A principal fonte das citações dos periódicos brasileiros provém de artigos nacionais, sendo fundamental conhecer o quanto eles referenciam artigos de periódicos brasileiros. Objetivo: Verificar a frequência com que artigos nacionais são citados nas referências de artigos de três periódicos brasileiros de fisioterapia. Método: Avaliaram-se todas as referências dos artigos publicados nos periódicos: Fisioterapia em Movimento, Fisioterapia e Pesquisa e Revista Brasileira de Fisioterapia nos anos de 2010 a 2012, verificando a quantidade de artigos provenientes de revistas nacionais e internacionais. Resultados: Foram analisadas 13.009 referências, dispostas em 456 artigos nas três revistas. Desse total, 2.924 (22,47%) foram de periódicos nacionais. Não houve diferença significativa entre os períodos analisados. Trinta e seis (7,89%) dos artigos não citaram artigo de periódico nacional, e 65 (14,25%) citaram mais artigos nacionais que estrangeiros. Conclusão: O valor médio da porcentagem de artigos nacionais citados nos artigos estudados foi de 22,47%. Não houve diferença entre os períodos estudados. .